Journal: Cell Biology and Toxicology

Article Title: BMSC-derived extracellular vesicles affect gluconeogenesis and lipogenesis by releasing 5'-tRF-GlyCCC to improve MAFLD insulin sensitivity

doi: 10.1007/s10565-025-10132-5

Figure Lengend Snippet: Identification of lipogenesis in PA-treated HepG2 cells. A The lipid accumulation was detected in different groups by Oil Red O staining. B The adipogenic protein expression levels of SREBP1c-FL (full-length), SREBP1c-N (N-terminal), FASN and SCD1 were detected by WB. C The quantification of WB data for the protein levels was normalized to GAPDH and expressed relative to the control group. D The secretion of triglyceride (TG) in HepG2 cells was measured using a TG detection kit. E The secretion of total cholesterol(TC) in HepG2 cells was measured using a TC detection kit. ** p <0.01, *** p <0.001, **** p <0.0001.

Article Snippet: The cells were subjected to probing using primary antibodies specifically targeting CD9 (1:1000, Abcam in the UK) and CD63 (1:800, Abcam).UK), ALIX (1:1000, USA), pFoxO3 (1:1000), FoxO3 (1:1000), PEPCK (1:1000), G6Pase (1:1000,Abcam (UK), SREBP1c-FL (1:1000, USA), and SREBP1c-N (1:1000, USA), FASN, SCD1 (1:1000), and GAPDH (1:5000, Proteintech, USA) for 12 h at 4°C.

Techniques: Staining, Expressing, Control

Journal: Cell Biology and Toxicology

Article Title: BMSC-derived extracellular vesicles affect gluconeogenesis and lipogenesis by releasing 5'-tRF-GlyCCC to improve MAFLD insulin sensitivity

doi: 10.1007/s10565-025-10132-5

Figure Lengend Snippet: Identification of lipogenesis in PA-treated HepG2 cells. A The lipid accumulation was detected in different groups by Oil Red O staining. B The adipogenic protein expression levels of SREBP1c-FL (full-length), SREBP1c-N (N-terminal), FASN and SCD1 were detected by WB. C The quantification of WB data for the protein levels was normalized to GAPDH and expressed relative to the control group. D The secretion of triglyceride (TG) in HepG2 cells was measured using a TG detection kit. E The secretion of total cholesterol(TC) in HepG2 cells was measured using a TC detection kit. ** p <0.01, *** p <0.001, **** p <0.0001.

Article Snippet: The cells were subjected to probing using primary antibodies specifically targeting CD9 (1:1000, Abcam in the UK) and CD63 (1:800, Abcam).UK), ALIX (1:1000, USA), pFoxO3 (1:1000), FoxO3 (1:1000), PEPCK (1:1000), G6Pase (1:1000,Abcam (UK), SREBP1c-FL (1:1000, USA), and SREBP1c-N (1:1000, USA), FASN, SCD1 (1:1000), and GAPDH (1:5000, Proteintech, USA) for 12 h at 4°C.

Techniques: Staining, Expressing, Control